Granuphilin c2a

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August undefined, 2024

WebOct 31, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its

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WebAug 24, 2024 · The C2A domain of granuphilin binds strongly to plasma membranes containing PIP2 via a conserved lysine cluster (red circle) embedded in a large electropositive surface (blue oval) containing many basic residues (blue sticks). A single exposed hydrophobic residue (gray sticks) also contributes to membrane affinity. WebGranuphilin C2A displays a lateral diffusion constant comparable to other C2 domains. However, the diffusion of the granuphilin C2AB tandem on the same membrane appears to be slower; suggesting substantial PS contacts for the C2B domain within the C2AB tandem. This effect represents a weak dexterous manipulation of cloth

Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2 ... - PubMed

WebOct 12, 2006 · Like granuphilin toward insulin granules, exophilin4 promotes the targeting of glucagon granules to the plasma membrane. Although the interaction of granuphilin with syntaxin-1a is critical for the targeting activity, exophilin4 does this primarily through the afﬁnity of its C2A domain toward the plasma membrane WebCorvallis Veterinary Hospital. Jul 2007 - Sep 20092 years 3 months. 1543 NW 9th St. Corvallis, OR 97330. As part of a small veterinary clinic I gained proficiency in a diverse set of skills ... WebDec 20, 2006 · Instead, the C2 domains, particularly the C2A domain, of exophilin4 showed markedly higher phospholipid-binding activities than those of granuphilin. The … church tithe software

Multivalent lipid targeting by the calcium-independent C2A …

WebJan 1, 2024 · The C2A domain of Slp-4 is known to drive this protein's ability to bind cellular PMs primarily via interaction with PIP 2 (12, 13).However, the protein retains an affinity for membranes containing the anionic lipids PS and/or PI, even in the absence of PIP 2 ().Here, we set out to discern the contributions of PIP 2 and background anionic lipid binding to … WebFeb 1, 2015 · The recognition of granuphilin C2A to PI(4,5)P2 within lipid bilayer was analyzed by NMR, using nanodisc system. Abstract. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a key player in regulating the process of excytosis, including insulin secretion. Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin ... dexterous thesaurusWebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … dexterous shadow weapon

"WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma … " - Granuphilin c2a

Granuphilin c2a

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WebJun 3, 2024 · Synaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before … Webusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding afﬁnity of this protein. This suggests that granuphilin may serve as a coincidence de-

WebJan 13, 2015 · Another example is the study of the interaction of granuphilin-C2A domain with phosphatidylinositol 4, 5-bisphosphate [PI(4, 5)P 2 ] head group and PI(4, 5)P 2 … WebGranuphilin is a membrane binding protein responsible, in part, for docking insulin secretory vesicles to the plasma membrane. Granuphilin, also known as synaptotagmin …

WebThe C2A domain plays key roles in this process through interaction with PI (4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A … WebRab27a and Rab27b have recently been recognized to play versatile roles in regulating the exocytosis of secretory granules and lysosome-related organelles by using multiple effector proteins. However, the precise roles of these effector proteins in particular cell types largely remain uncharacterized, except for those in pancreatic β cells and in melanocytes. Here, …

WebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2. 7: 24184645: 2014: The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids. 5: 27032672: 2016: Granuphilin exclusively mediates functional granule docking to the plasma membrane. 4:

WebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … church tithes and offering envelopesWebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma … dexterous shieldWebFeb 15, 2006 · Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a is specifically localized on dense-core vesicles in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. However, the precise molecular mechanism of its inhibitory effect on exocytosis has never been … dexterous visitor helmetWebApr 1, 2016 · Furthermore, granuphilin forms a 180-nm cluster at the site of each docked granule, along with granuphilin-interacting Rab27a and Munc18-1 clusters. These … dexterous shadowWebMar 7, 2002 · Granuphilin/Slp-4 is a member of the synaptotagmin-like protein family expressed in pancreatic β-cells and in the pituitary gland. We show by confocal microscopy that both granuphilin-a and -b colocalize with insulin-containing secretory granules positioned at the periphery of pancreatic β-cells. Overexpression of granuphilins in … dexterous underwater robot hand: heu hand iiWebDec 12, 2013 · Proteins containing C2 domains are the sensors for Ca 2+ and PI(4,5)P 2 in a myriad of secretory pathways. Here, the use of a free-mounting system has enabled us to capture an intermediate state of Ca 2+ binding to the C2A domain of rabphilin 3A that suggests a different mechanism of ion interaction.Structural comparisons between C2A … dexterous person meaningWebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma membrane cytosolic leaflet (Corbin et al., 2007, Voelker, 2008).Since some C2 domains are known to dock preferentially to PIP x lipids, such physiologically relevant lipid mixtures … dexterous shot lost ark