Ateh1/pan1

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August undefined, 2024

WebDec 5, 2024 · The authors combine X-ray crystallography, NMR and MD simulations with biochemical and in planta analysis to characterize the two AtEH1/Pan1 EH domains and reveal their structural differences and complementary functional roles. 16 PDF View 2 excerpts, cites background A historical perspective on the regulation of cellulose …

TPLATE Complex Subunits Are Recruited Simultaneously to the …

WebIn particular, two TPC members, AtEH1/Pan1 and AtEH2/Pan1 play an important role in this pathway (Figure 8) and several components of the endocytic machinery are associated with this pathway, either as machinery or as cargo. Ongoing research focusses on unravelling in detail how this pathway is initiated, as well as how it is connected to the ... WebMar 25, 2024 · RCSB PDB - 6YET: Second EH domain of AtEH1/Pan1 Structure Summary NMR Ensemble 3D View : Structure 1D-3D View Validation Report Ligand Interaction Macromolecule Content Total Structure Weight: 12.38 kDa Atom Count: 858 Modelled Residue Count: 104 Deposited Residue Count: 106 Unique protein chains: 1 Display … great american taco company springfield mo

Autophagosome Biogenesis in Plants: An Actin ... - ScienceDirect

WebUtilizing advanced time-lapse imaging and kymograph analysis, the authors show that the TPLATE, AtEH1/Pan1, and AtEH2/Pan1 TPC subunits are recruited to the PM simultaneously, at both 25°C and 12°C ( Fig. 1 ). WebThe first EH domain of AtEH1/Pan1 interacts with the N-terminal double NPF motif of SCAMP5. a, Graphical representation of SCAMP5, the double N-terminal NPF motif is … WebApr 9, 2024 · The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endo-cytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homo-logues of the yeast ARP2/3 ... great american takeout day

The TPLATE complex mediates membrane bending …

Distinct EH domains of the endocytic TPLATE complex …

WebNov 13, 2024 · The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. … WebSep 1, 2024 · AtEH1/Pan1 contains a typical VCA motif, which is responsible for ARP2/3 activation; it is able to bind to actin filaments and interact with APR2/3 subunits, suggesting a similar NPF function in plants. AtEH/Pan1 localizes to autophagosomes and promotes endosome to autophagosome conversion (Figure 2 B). great american tape companyWebMar 18, 2024 · The authors combine X-ray crystallography, NMR and MD simulations with biochemical and in planta analysis to characterize the two AtEH1/Pan1 EH domains and reveal their structural differences and complementary functional roles. Expand 16 Highly Influential PDF View 24 excerpts, references background, methods and results ... 1 2 3 4 … choosing raw recipes

"WebThe Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are … " - Ateh1/pan1

Ateh1/pan1

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Web6YEU: Second EH domain of AtEH1/Pan1. You are using a web browser that we do not support. Our website will not work properly. WebThe TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and …

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WebApr 23, 2024 · Collectively, our structural and functional studies reveal distinct but complementary roles of the EH domains of AtEH/Pan1 in plant CME and connect the internalization of SCAMP5 to the TPLATE complex. AtEH/Pan1 proteins contain two N-terminal Eps15 homology (EH) domains and are subunits of the endocytic TPLATE … WebMay 24, 2024 · The AtEH/Pan1 proteins are the plant homologs of yeast Pan1p, which is known for its role as an activator of ARP2/3-dependent actin dynamics during endocytosis 9 – 11. Pan1p was also recently shown to be part of a phosphorylation-dependent mechanism connecting endocytic vesicles, endosomal compartments, and actin dynamics in budding …

WebNov 13, 2024 · Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway … WebJan 18, 2024 · The actin cytoskeleton interacts with multiple membrane compartments and regulates a plethora of subcellular responses, including cell growth, cell wall deposition and organelle positioning (Kadota et al ., 2009; Whippo et al ., 2011; Lanza et al ., 2012; Henty-Ridilla et al ., 2013; Sassmann et al ., 2024; Wang et al ., 2024; Hawkins et al ., …

WebBefore cross-linking, TML and AtEH1/Pan1 subunits were expressed in Arabidopsis cell cultures grown in continous dark (Plant Systems Biology-Dark; PSB-D) with a C-terminal … WebMar 17, 2024 · Here we used live-cell imaging and biochemical approaches to investigate the function of the Arabidopsis thaliana TPC subunit AtEH1/Pan1. Using in vitro and in …

WebNov 13, 2024 · AtEH1/Pan1 and AtEH2/Pan1 are essential for plant life. In AtEH1/Pan1 heterozygous mutants, shrivelled and normal looking pollen grains are observed in a …

WebAtrium Health Carolinas Medical Center. 1000 Blythe Blvd. Charlotte, NC 28203. Phone: 704-355-2000. Atrium Health Mercy, a facility of Carolinas Medical Center. 2001 Vail Ave. Charlotte, NC 28207. Phone: 704-304-5000. choosing recording buffer size for videosWebTPLATE, along with the AtEH/Pan1 subunits, has been shown to play a major role in these intermolecular protein-protein interactions. Interactions among the TPC subunits remain, however, scarce. Next to its function as a protein interaction hub, TPC localizes to membranes to fulfill its role. great american taco springfield moWebDec 14, 2024 · The two EH domains of atEH1, EH1.1 and EH1.2 as defined by ref. 21, were amplified from synthetic AtEH1/Pan1 (codon optimized for bacterial expression, IDT) (SI … choosing realtorWebMar 18, 2024 · Clathrin-mediated endocytosis (CME) is an essential cellular internalisation pathway involving the dynamic assembly of clathrin and accessory proteins to form … great american talk radioWebYeast transformed with pGBKT7-S7-2/pGADT7 or pGBKT7/pGADT7-SKP1 Maize used as negative controls were unable to grow. Techniques Used: Positive Control, Sequencing, Construct, Transformation Assay control plasmids pgbkt7 p53 ( TaKaRa ) TaKaRa is a verified supplier TaKaRa manufactures this product About News Press Release Team … choosing reclaimed wood flooringWebMoreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either … choosing reclinersWebDec 1, 2024 · Transgenic lines expressing AtEH1/Pan1-mRuby3 in Col-0 and (ateh1 pan1 1-2 −/− backgrounds also exhibited a band for AtEH1/Pan1-mRuby3 at ~175 kDa (Extended Data Fig. 3d, marked by white ... great american tail